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BiosearchTech: New Blog Article! Molecular Beacons - Lights in the storm http://bit.ly/bgqCm2

Wed, 10 Mar 2010 19:15:07 +0000
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Fluorescence Detection of Single-Nucleotide Polymorphisms with a Single, Self-Complementary, Triple-Stem DNA Probe.
Xiao, Y., Plakos, K., Lou, X., White, R., Qian, J., Plaxco, K., Soh, H. Angewandte Chemie. 2009, 48(24) 4354-4358.

Label-Free Electrochemical Detection of DNA in Blood Serum via Target-Induced Resolution of an Electrode-Bound DNA Pseudoknot. Xiao, Y., Qu, X., Plaxco, K.W., and Heeger, A.J. J. Am. Chem. Soc. 2007, 129, 11896-11897.

Hypusination of eukaryotic initiation factor 5A (eIF5A): a novel therapeutic target in BCR-ABL-positive leukemias identified by a proteomics approach. Balabanov S, Gontarewicz A, Ziegler P, Hartmann U, Kammer W, Copland M, Brassat U, Priemer M, Hauber I, Wilhelm T, Schwarz G, Kanz L, Bokemeyer C, Hauber J, Holyoake TL, Nordheim A, Brümmendorf TH. Blood 2007, 109, 1701–1711.

Optical Antisense Imaging of Tumor with Fluorescent DNA Duplexes Liu, X.; Wang, Y; Nakamura, K; Liu, G; Dou, S; Kubo, A; Rusckowski, M; Hnatowich, D.J. Bioconjugate Chemistry 2007, 18, 1905–1911.

Quantification of mRNA using real-time RT-PCR. Nolan, T., Hands, R.E., Bustin, S.A. Nature Protocol. 2006, 1(3), 1559-1582.

Development and application of a quantitative RT-PCR potency assay for a pentavalent rotavirus vaccine (RotaTeq©) Ranheim, T.; Mathis, P.K.; Joelsson, D.B.; Smith, M.E.; Campbell, K.M.; Lucas, G.; Barmat, S.; Melissen, E.; Benz, R.; Lewis, J.A.; Chen, J.; Schofield, T.; Sitrin, R.D.; Hennessey, J.P. Journal of Virological Methods 2006, 131, 193-201.

2'-Deoxy-2'-Fluoro-á-D-Arabinonucleic Acid (2'F-ANA) Modified Oligonucleotides (ON) Effect Highly Efficient, and Persistent, Gene Silencing Kalota, A.; Karabon, L.; Swider, C.R.; Viazovkina, E.; Elzagheid, M.; Damha, M.J.; Gewirtz, A.M. Nucleic Acids Research. 2006, 34, 451 461.

Live-Cell Characterization and Analysis of a Clinical Isolate of Bovine Respiratory Syncytial Virus, Using Molecular Beacons Santangelo, P.; Nitin N.; Laconte, L.; Woolums, A.; Bao, G. J. Virol. 2006, 80, 682 688.





Age-Dependent Dopaminergic Dysfunction in Nurr1 Knockout Mice Jiang. C.; Wan X.; He Y.; Pan T.; Jankovic J.; Le W. Exp Neurol 2005, 191, 154 162.

Use of Molecular Beacons for Rapid, Real-Time, Quantitative Monitoring of Cytotoxic T-Lymphocyte Epitope Mutations in Simian Immunodeficiency Virus Peyerl, F.W.; Barouch, D.H.; Bazick, H.S.; Manuel, E.; Letvin. N.L. Journal of Clinical Microbiology 2005, 43, 4773-4779.

Analysis of Repetitive Element DNA Methylation by MethyLight Weisenberger D.J.; Campan M.; Long T.I.; Kim M.J.; Woods C.; Fiala E.; Ehrlich M.; Laird P.W. Nucleic Acids Research 2005, 33, 6823-6836.

Sensitive Detection of Human Papillomavirus in Cervical, Head/Neck, and Schistosomiasis-Associated Bladder Malignancies Yang, H.; Yang, K.; Khafagi, A.; Tang, Y.; Carey, T.E.; Opipari, A.W,; Lieberman, R.; Oeth, P.A.; Lancaster, W.; Klinger, HP.; Kaseb, A.O.; Metwally, A.; Khaled, H.; Kurnit, D.M. PNAS 2005, 102, 7683-7688.

Quantification of Toxic Microcystis spp. During The 2003 and 2004 Blooms In Western Lake Erie Using Quantitative Real-Time PCR Rinta-Kanto, J.M.; Ouellette, A.J.A.; Boyer, G.L.; Twiss, M.R.; Bridgeman, T.B.; Wilhelm, S.W. Environ. Sci. Technol. 2005, 39, 4198-4205.

Interleukin-1 Decreases Expression of the Epithelial Sodium Channel -Subunit in Alveolar Epithelial Cells via a p38 MAPK-dependent Signaling Pathway Roux, J.; Kawakatsu, H.; Gartland, B.; Pespeni, M.; Sheppard, D.; Matthay. M.A.; Canessa. C.M.; Pittet. J.F. J. Biol. Chem. 2005, 280, 18579-18589.


Quantification of HTLV-I proviral load in experimentally infected rabbits Zhao, T.M.; Hague, B.; Caudell, D.L.; Simpson, R.M.; Kindt, T. J. Retrovirology 2005, 34, .

Prenatal Exposure To Ethanol Causes Partial Diabetes Insipidus In Adult Rats Knee, D.S.; Sato, A.K.; Uyehara, C.F.T.; Claybaugh, J.R. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2004, 287, 277-283.

Tumor Necrosis Factor-Alpha Inhibits Peroxisome Proliferator-Activated Receptor Gamma Activity At A Posttranslational Level In Hepatic Stellate Cells Sung, C.K.; She, H.; Xiong, S.; Tsukamoto, H. Am. J. Physiol. Gastrointest. Liver. Physiol. 2004, 286, G722-G729.

Rewritable Memory By Controllable Nanopatterning Of DNA Shin, J.S.; Pierce, N.A. Nano Letters 2004, 4, 905-909.

IL-6 Mediates Hypoferremia Of Inflammation By Inducing The Synthesis Of The Iron Regulatory Hormone Hepcidin Nemeth, E.; Rivera, S.; Gabayan, V.; Keller, C.; Taudorf, S.; Pedersen, B.K.; Ganz, T. J. Clin. Invest. 2004, 113, 1271-1276.

High-Throughput Analysis Of Oligonucleotides Using Automated Electrospray Ionization Mass Spectrometry Hail, M.B.; Elliott, B.; Anderson, K. Am. Biotechnol. Lab. 2004, 22, 12-14.

Homogeneous Time-Resolved Fluorescence Quenching Assay (Trupoint) For Nucleic Acid Detection Ylikoski, A.; Elomaa, A.; Ollikka, P; Hakala, H.; Mukkala, V.M.; Hovinen, J.; Hemmila, I. Clin. Chem. 2004, 50, 1943-1947.

Enhancement Of Collecting Duct Renin In Angiotensin II|Dependent Hypertensive Rats Prieto-Carrasquero, M.C.; Harrison-Bernard, L.M.; Kobori, H.; Ozawa, Y.; Hering-Smith, K.S.; Hamm, L.L; Navar, L.G. Hypertension 2004, 44, 223-229.

Dermal Papilla-Induced Hair Differentiation Of Adult Epithelial Stem Cells From Human Skin Roh, C.; Tao, Q.; Lyle, S. Physiol. Genomics 2004, 19, 207-217.

Transgenic Rescue Demonstrates Involvement Of The Ian5 Gene In T Cell Development In The Rat Michalkiewicz, M.; Michalkiewicz, T.; Ettinger, R.A.; Rutledge, E.A.; Fuller, J.M.; Moralejo, D.H.; Van Yserloo, B.; MacMurray, A.J.; Kwitek, A.E.; Jacob, H.J.; Lander, E.S.; Lernmark, A. Physiol. Genomics 2004, 19, 228-232.

A Real-Time PCR Assay For DNA-Methylation Using Methylation-Specific Blockers Cottrell, S.E.; Distler, J.; Goodman, N.S., Mooney, S.H.; Kluth, A.; Olek, A.; Schwope, I.; Tetzner, R.; Ziebarth, H.; Berlin, K. Nucl. Acids Res. 2004, 32, e10.

Single Cell Analysis Of Switch-Like Induction Of CYP1A1 In Liver Cell Lines Broccardo, C.J.; Billings, R.E.; Chubb, L.S.; Andersen, M.E.; Hanneman, W.H. Toxicol. Sci. 2004, 78, 287-294.

Predominance Of Th1 Cytokine In Peripheral Blood And Pathological Tissues Of Patients With Active Untreated Adult Onset Still's Disease Chen, D.Y.; Lan, J.L.; Lin, F.J.; Hsieh, T.Y.; Wen, M.C. Ann. Rheum. Dis. 2004, 63, 1300-1306.

Kin Cohort Estimates For Familial Breast Cancer Risk In Relation To Variants To DNA Base Excision Repair, BRAC1 Interacting And Growth Factor Genes Sigurdson, A.J.; Hauptmann, M.; Chatterjee, N.; Alexander, B.H.; Doody, M.M.; Rutter, J.M.; Struewing, J.P. BMC Cancer 2004, 4, 9.

IL-15-Independent Proliferative Renewal Of Memory CD8 T Cells In Latent Gammaherpesvirus Infection Obar, J.J.; Crist, S.G.; Leung, E.K.; Usherwood, E.J. J. Immunol. 2004, 173, 2705-2714.

Fast Track Inhibition Of MUC1 Expression By Indole-3-Carbinol Lee, J.; Han, J; Baek, J.; Hisatsune, A.; Kim, K.C. Int. J. Cancer 2004, 109, 810-816.

Attenuation Of Th1 Effector Cell Responses And Susceptibility To Experimental Allergic Encephalomyelitis In Histamine H2 Receptor Knockout Mice Is Due To Dysregulation Of Cytokine Production By Antigen-Presenting Cells Teuscher, C.; Poynter, M.E.; Offner, H.; Zamora, A.; Watanabe, T.; Fillmore, P.D.; Zachary, J.F.; Blankenhorn, E.P. Am. J. Pathol. 2004, 164, 883-892.


Increased CD36 Protein As A Response To Defective Insulin Signaling In Macrophages Liang, C.; Han, S.; Okamoto, H.; Carnemolla, R.; Tabas, I.; Accili, D.; Tall, A.R. J. Clin. Invest. 2004, 113, 764-773.

Whole Genome Scanning Identifies Genotypes Associated With Recurrence And Metastasis In Prostate Tumors Paris, P.L.; Andaya, A.; Fridlyand, J.; Jain, A.N.; Weinberg, V.; Kowbel, D.; Brebner, J.H.; Simko, J.; Watson, J.E.V.; Volik, S.; Albertson, D.G.; Pinkel, D.; Alers, J.C.; van der Kwast, T.H.; Vissers, K.J.; Schroder, F.H.; Wildhagen, M.F.; Febbo, P.G.; Chinnaiyan, A.M.; Pienta, K.J.; Carroll, P.R.; Rubin, M.A.; Collins, C.; van Dekken, H. Hum. Mol. Genet. 2004, 13, 1303-1313.

Deletion Of Microsomal Prostaglandin E2 (PGE2) Synthase-1 Reduces Inducible And Basal PGE2 Production And Alters The Gastric Prostanoid Profile Boulet, L.; Ouellet, M.; Bateman, K.P.; Ethier, D.; Percival, M.D.; Riendeau, D.; Mancini, J.A.; Methot, N. J. Biol. Chem. 2004, 279, 23229-23237.


Induction Of CYP1A1 In Primary Rat Hepatocytes By 3,3',4,4',5-Pentachlorobiphenyl: Evidence For A Switch Circuit Element French, C.T.; Hanneman, W.H.; Chubb, L.S.; Billings, R.E.; Andersen, M.E. Toxicol. Sci. 2004, 78, 276-286.

Mouse Skin Passage Of Streptococcus Pyogenes Results In Increased Streptokinase Expression And Activity Rezcallah, M.S.; Boyle, M.D.P.; Sledjeski, D.D. Microbiology 2004, 150, 365-371.

Acute Alcohol Inhibits TNF-{Alpha} Processing In Human Monocytes By Inhibiting TNF/TNF-{Alpha}-Converting Enzyme Interactions In The Cell Membrane Zhao, X.; Marrero, L.; Song, K.; Oliver, P.; Chin, S.Y.; Simon, H.; Schurr, J.R.; Zhang, Z.; Thoppil, D.; Lee, S.; Nelson, S.; Kolls, J.K. J. Immunol. 2003, 170, 2923-2931.

Rasgrp4 Regulates The Expression Of Prostaglandin D2 In Human And Rat Mast Cell Lines Li, L.; Yang, Y.; Stevens, R.L. J. Biol. Chem. 2003, 278, 4725-4729.

Myd88 Primers Macrophages For Full-Scale Activation By Interferon-Gamma Yet Mediates Few Response To Mycobacterium Tuberculosis Shi, S.; Nathan, C.; Schnappinger, D.; Drenkow, J.; Fuortes, M.; Block, E.; Ding, A.; Gingeras, T.R.; Schoolnik, G.; Akira, S.; Takeda, K.; Ehrt, S. J. Exp. Med. 2003, 198, 987-997.

Use Of Real-Time PCR To Detect And Quantify Mycoplasma Haemofelis And Candidatus Mycoplasma Haemominutum DNA Tasker, S.; Helps, C.R.; Day, M.J.; Gruffydd-Jones, T.J.; Harbour, D.A. J. Clin. Microbiol. 2003, 41, 439-441.



Short Communication: Prevalence Of Entamoeba Histolytica And Entamoeba Dispar In Northern Ghana Verweij, J.; Oostvogel, F.; Brienen, E.A.T.; Nang-Beifubah, A.; Ziem, J.; Polderman, A.M. Tropical Medicine International Health 2003, 8, 1153-1156.

Multiplex Real-Time PCR Method To Identify Shiga Toxin Genes Stx1 And Stx2 And Escherichia Coli O157:H7/H-Serotype Jinneman, K.C.; Yoshitomi, K.J.; Weagant, S.D. Appl. Environ. Microbiol. 2003, 69, 6327-6333.

Ras GRP4 Regulates The Expression Of Prostaglandin D 2 In Human And Rat Mast Cell Lines Li, L.; Yang, Y.; Stevens, R.L. J. Biol. Chem. 2003, 278, 4725-4729.

Applications Of Competitor RNA In Diagnostic Reverse Transcription-PCR Kleiboeker, S.B. J. Clin. Microbiol. 2003, 41, 2055-2061.


Development And Clinical Evaluation Of An Internally Controlled, Single-Tube Multiplex Real-Time PCR Assay For Detection Of Legionella Pneumophila And Other Legionella Species Templeton, K.E.; Scheltinga, S.A.; Sillekens, P.; Crielaard, J.W.; van Dam, A.P.; Goossens, H.; Claas, E.C.J. J. Clin. Microbiol. 2003, 41, 4016-4021.


Evaluation Of Real-Time PCR For Detection Of And Discrimination Between Bordetella Pertussis, Bordetella Parapertussis, And Bordetella Holmesii For Clinical Diagnosis Templeton, K.E.; Scheltinga, S.A.; van der Zee, A.; Diederen, B.M.W.; Kruijssen, A.M.; Goossens, H.; Kuijper, E.; Claas, E.C.J. J. Clin. Microbiol. 2003, 41, 4121-4126.


Real-Time Polymerase Chain Reaction Wilhelm, J.; Pingoud, A. ChemBioChem 2003, 4, 1120-1128.

A novel Dual-labeled real-time PCR assay to estimate ex vivo human immunodeficiency virus type 1 fitness in the era of multi-target (pol and env) antiretroviral therapy Weber, J.; Rangel, H.R.; Chakraborty, B.; Tadele, M.; Martinez, M.A.; Martinez-Picado, J.; Marotta, M.L.; Mirza, M.; Ruiz, L.; Clotet, B.; Wrin, T.; Petropoulos, C.J.; Quinones-Mateu, M.E. J. Gen. Virol. 2003, 84, 2217-2228.


Transcriptional Silencing Of Zinc Finger Protein 185 Identified By Expression Profiling Is Associated With Prostate Cancer Progression Vanaja, D.K.; Cheville, J.C.; Iturria, S;J.; Young, C.Y.F. Can. Res. 2003, 63, 3877-3882.

Frequent Sampling Reveals Dynamic Responses By The Transcriptome To Routine Media Replacement In Hepg2 Cells Morgan, K.T.; Casey, W.; Easton, M.; Creech, D.; Ni, H. Toxicologic Pathology 2003, 31, 448-461.

Hypoxia-Induced Gene Expression Occurs Solely Through The Action Of Hypoxia-Inducible Factor 1{Alpha} (HIF-1{Alpha}): Role Of Cytoplasmic Trapping Of HIF-2{Alpha} Park, S.; Dadak, A.M.; Haase, V.H.; Fontana, L.; Giaccia, A.J.; Johnson, R.S. Mol. and Cell. Biol. 2003, 23, 4959-4971.

Streptococcus Pyogenes Infection In Mouse Skin Leads To A Time-Dependent Up-Regulation Of Protein H Expression Smith, T.C.; Sledjeski, D.D.; Boyle, M.D.P. Infect. Immun. 2003, 71, 6079-6082.

The Hag Protein Of Moraxella Catarrhalis Strain O35E Is Associated With Adherence To Human Lung And Middle Ear Cells Holm, M.M.; Vanlerberg, S.L.; Sledjeski, D.D.; Lafontaine, E.R. Infect. Immun. 2003, 71, 4977-4984.

Genetic Analysis Of The Influence Of Neuroantigen-Complete Freund's Adjuvant Emulsion Structures On The Sexual Dimorphism And Susceptibility To Experimental Allergic Encephalomyelitis Fillmore, P.D.; Brace, M.; Troutman, S.A.; Blankenhorn, E.P.; Diehl, S.; Rincon, M.; Teuscher, C. Am. J. Pathol. 2003, 163, 1623-1632.

DNA Damage Response And MCL-1 Destruction Initiate Apoptosis In Adenovirus-Infected Cells Cuconati, A.; Mukherjee, C.; Perez, D.; White, E. Genes Dev. 2003, 17, 2922-2932.

HMGA2 Expression In Uterine Leiomyomata And Myometrium: Quantitative Analysis And Tissue Culture Studies Gross, K.L.; Neskey, D.M.; Manchanda, N.; Weremowicz, S.; Kleinman, M.S.; Nowak, R.A.; Ligon, A.H.; Rogalla, P.; Drechsler, K.; Bullerdiek, J.; Morton, C.C. Genes Chromosomes Cancer 2003, 38, 68-79.

Nerve Growth Factor Blocks The Glucose-Induced Down-Regulation Of Caveolin-1 Expression In Schwann Cells Via P75 Neurotrophin Receptor Signaling Tan, W.; Rouen, S.; Barkus, K.M.; Dremina, Y.S.; Hui, D.; Christianson, J.A.; Wright, D.E.; Yoon, S.O.; Dobrowsky, R.T. J. Biol. Chem. 2003, 278, 23151-23162.

RNA Interference-Mediated Knockdown Of A GATA Factor Reveals A Link To Anautogeny In The Mosquito Aedes Aegypti Attardo, G.M.; Higgs, S.; Klingler, K.A.; Vanlandingham, D.L.; Raikhel, A.S. PNAS 2003, 100, 13374-13379.


Acute Alcohol Inhibits TNF-{Alpha} Processing In Human Monocytes By Inhibiting TNF/TNF-{Alpha}-Converting Enzyme Interactions In The Cell Membrane Zhao, X.; Marrero, L.; Song, K.; Oliver, P.; Chin, S.Y.; Simon, H.; Schurr, J.R.; Zhang, Z.; Thoppil, D.; Lee, S.; Nelson, S.; Kolls, J.K. J. Immunol. 2003, 170, 2923-2931.

Real-Time PCR For Quantification Of Giardia And Cryptosporidium In Environmental Water Samples And Sewage Guy, R.A.; Payment, P.; Krull, U.J.; Horgen, P.A. Appl. Environ. Microbiol. 2003, 69, 5178-5185.

Gene Expression Profiling Reveals Progesterone-Mediated Cell Cycle And Immunoregulatory Roles Of Hoxa-10 In The Preimplantation Uterus Yao, M.W.M.; Lim, H.; Schust, D.J.; Choe, S.E.; Farago, A.; Ding, Y.; Michaud, S.; Church, G.M.; Maas, R.L. Mol. Endocrinol. 2003, 17, 610-627.

Cytokine-Sensitive Replication Of Hepatitis B Virus In Immortalized Mouse Hepatocyte Cultures Pasquetto, V.; Wieland, S.F.; Uprichard, S.L.; Tripodi, M.; Chisari, F.V. J. Virol. 2003, 76, 5646-5653.



Multiplex Fluorogenic Real-Time PCR For Detection And Quantification Of Escherichia Coli O157:H7 In Dairy Wastewater Wetlands Ibekwe, A.M.; Watt, P.M.; Grieve, C.M.; Sharma, V.K.; Lyons, S.R. Appl. Environ. Microbiol. 2002, 68, 4853-4862.



A Mutant Epidermal Growth Factor Receptor Targeted To Lung Epithelium Inhibits Asbestos-Induced Proliferation And Proto-Oncogene Expression Manning, C.B.; Cummins, A.B.; Jung, M.W.; Berlanger, I.; Timblin, C.R.; Palmer, C.; Taatjes, D.J.; Hemenway, D.; Vacek, P.; Mossman, B.T. Can. Res. 2002, 62, 4169-4175.


Latent Antigen Vaccination In A Model Gammaherpesvirus Infection Usherwood, E.J.; Ward, K.A.; Blackman, M.A.; Stewart, J.P.; Woodland, D.L. J. Virol. 2001, 75, 8283-8288.

A Novel Method Of Gene Transcript Profiling In Airway Biopsy Homogenates Reveals Increased Expression Of A Na+-K+-Cl- Cotransporter (NKCC1) In Asthmatic Subjects Dolganov, G.M.; Woodruff, P.G.; Novikov, A.A.; Zhang, Y.; Ferrando, R.E.; Szubin, R.; Fahy, J.V. Genome Res. 2001, 11, 1473-1483.