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BiosearchTech: New Blog Article! Factors affecting fluorophore performance in qPCR http://t.co/imulE1JH
1/11/2012 8:56:07 AM
BiosearchTech: New Blog Article! Factors affecting fluorophore performance in qPCR http://t.co/imulE1JH
1/11/2012 8:56:07 AM
DNA Modification Reagents |
Biosearch offers a broad range on non-fluorescing modifiers for popular oligonucleotide applications. Amidites are used for 5’ or internal modification, and Columns and CPGs are used for 3’ modification of synthetic oligonucleotides.
Amino Modifying Amidites
Used to add amino functionality to oligonucleotides, either within the oligo or on the 5’ terminus.
Amino Modifying Columns & CPGs
Reagents for incorporating a functionalized primary amine on the 3’ terminus of the oligonucleotide.
Biotinylating Amidites
Used to label the 5’ end or place an internal biotin label in synthetic oligonucleotides for subsequent detection through enzyme-labeled streptavidin or streptavidin conjugates.
Biotinylating Columns & CPGs
Used for the synthesis of 3’ biotin-labeled oligonucleotides.
Phosphorylating Amidites
Used for placing a 5’-phosphate group on synthetic oligonucleotides. Applications are gene construction, cloning, mutagenesis and ligation chain reaction.
Phosphorylating Columns & CPGs
These reagents allow the preparation of synthetic oligonucleotides with a 3’ Phosphate group using standard synthesis protocols.
Thiol Modifier Amidites
These amidites are used to functionalize the 5’ terminus of an oligonucleotide with a thiol group, allowing attachment to fluorescent dyes, makeimide compounds or conjugation to proteins via disulfide linkages.
Thiol Modifier CPGs
This CPG is used to functionalize the 3’ terminus of an oligonucleotide with a thiol group.
Spacer Modifier Amidites
These amidites incorporate spacers into synthetic oligonucleotides, and may be added sequentially to create longer spacers.
Spacer Modifier Columns & CPGs
These reagents allow the addition of spacers at the 3’ end of an oligonucleotide. Spacers at the 3’ end inactivate the 3’ OH toward enzymatic processing, and may be used in lieu of 3’ phosphate groups.
Other Modifying Columns & CPGs
Used to add amino functionality to oligonucleotides, either within the oligo or on the 5’ terminus.
Amino Modifying Columns & CPGs
Reagents for incorporating a functionalized primary amine on the 3’ terminus of the oligonucleotide.
Biotinylating Amidites
Used to label the 5’ end or place an internal biotin label in synthetic oligonucleotides for subsequent detection through enzyme-labeled streptavidin or streptavidin conjugates.
Biotinylating Columns & CPGs
Used for the synthesis of 3’ biotin-labeled oligonucleotides.
Phosphorylating Amidites
Used for placing a 5’-phosphate group on synthetic oligonucleotides. Applications are gene construction, cloning, mutagenesis and ligation chain reaction.
Phosphorylating Columns & CPGs
These reagents allow the preparation of synthetic oligonucleotides with a 3’ Phosphate group using standard synthesis protocols.
Thiol Modifier Amidites
These amidites are used to functionalize the 5’ terminus of an oligonucleotide with a thiol group, allowing attachment to fluorescent dyes, makeimide compounds or conjugation to proteins via disulfide linkages.
Thiol Modifier CPGs
This CPG is used to functionalize the 3’ terminus of an oligonucleotide with a thiol group.
Spacer Modifier Amidites
These amidites incorporate spacers into synthetic oligonucleotides, and may be added sequentially to create longer spacers.
Spacer Modifier Columns & CPGs
These reagents allow the addition of spacers at the 3’ end of an oligonucleotide. Spacers at the 3’ end inactivate the 3’ OH toward enzymatic processing, and may be used in lieu of 3’ phosphate groups.
Other Modifying Columns & CPGs
