Fluorogenic Probes and Primers |
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Biosearch Technologies offers a variety of probe and primer formats for real-time qPCR gene expression assays and SNP genotyping. As inventors of the Black Hole Quencher® (BHQ®) dyes, every probe format is available as a BHQ dye labeled oligo. In addition, each oligo may be labeled with a variety of other fluorophores such as FAM, TET, HEX, JOE, CAL Fluor®, Quasar®, and Pulsar® dyes. Other modifications are also available. View our Dye Selection Chart to see a 
list of dyes offered at Biosearch Technologies.
- View our probe formats animation (Flash plug-in required)
- Download our probe comparison chart (PDF)
- Learn the difference between static and FRET quenching
- Read our Editorial: Real-time PCR Diagnostics in the Pharmaceutical Industry (PDF)
Dual-Labeled BHQ® Probes
View Biosearch Technologies' wide selection of fluorophore-quencher combinations for dual-labeled probes. We synthesize high-quality, HPLC purified dual-labeled probes and ensure every order meets our purity stringency requirements by mass spectroscopy before we ship for delivery.
BHQplus™ Probes
Biosearch Technologies offers a new and advanced probe technology for qPCR - the BHQplus probe. The BHQplus probe is a compact probe fortified with our signature duplex stabilizing technology which enhances target specificity and maintains melting temperatures.
Molecular Beacons
Molecular Beacons have short complementary sequences which hybridize to form a stem-loop structure that holds the fluorophore and quencher close together in space. Biosearch Technologies currently offers Molecular Beacons labeled with either a Black Hole Quencher® or a dabcyl dye. These may be paired with a variety of reporter fluorophore molecules.
Scorpions® Primers
Scorpions® primers combines the probe and primer into one molecule and uses a uni-molecular mechanism that does not require enzymatic cleavage of the probe during PCR cycling. View Biosearch Technologies' selection of Scorpions primers.
Amplifluor® Primers
Amplifluor® primers combine primer and probe in one molecule and are often used for SNP/mutation detection studies. View Biosearch Technologies' selection of Amplifluor primers.
Plexor® Primers
With Plexor primers, in contrast to other PCR probe technologies, the accumulation of product results in a reduction in fluorescence. View Biosearch Technologies' current selection of Plexor primers.
Calibration and Reference Dyes
Trying to multiplex using FAM, CAL Fluor® and/or Quasar® dyes? Remember to optimize your instrument's performance by using calibration standards offered by Biosearch Technologies.
SuperROX® Reference Dye
SuperROX is a highly stable ROX passive reference dye that completely eliminates "ROX drop" and can be used when a water soluble version of ROX is required as a passive reference for normalization of fluorescent signals in real-time PCR.
View Biosearch Technologies' wide selection of fluorophore-quencher combinations for dual-labeled probes. We synthesize high-quality, HPLC purified dual-labeled probes and ensure every order meets our purity stringency requirements by mass spectroscopy before we ship for delivery.
BHQplus™ Probes
Biosearch Technologies offers a new and advanced probe technology for qPCR - the BHQplus probe. The BHQplus probe is a compact probe fortified with our signature duplex stabilizing technology which enhances target specificity and maintains melting temperatures.
Molecular Beacons
Molecular Beacons have short complementary sequences which hybridize to form a stem-loop structure that holds the fluorophore and quencher close together in space. Biosearch Technologies currently offers Molecular Beacons labeled with either a Black Hole Quencher® or a dabcyl dye. These may be paired with a variety of reporter fluorophore molecules.
Scorpions® Primers
Scorpions® primers combines the probe and primer into one molecule and uses a uni-molecular mechanism that does not require enzymatic cleavage of the probe during PCR cycling. View Biosearch Technologies' selection of Scorpions primers.
Amplifluor® Primers
Amplifluor® primers combine primer and probe in one molecule and are often used for SNP/mutation detection studies. View Biosearch Technologies' selection of Amplifluor primers.
Plexor® Primers
With Plexor primers, in contrast to other PCR probe technologies, the accumulation of product results in a reduction in fluorescence. View Biosearch Technologies' current selection of Plexor primers.
Calibration and Reference Dyes
Trying to multiplex using FAM, CAL Fluor® and/or Quasar® dyes? Remember to optimize your instrument's performance by using calibration standards offered by Biosearch Technologies.
SuperROX® Reference Dye
SuperROX is a highly stable ROX passive reference dye that completely eliminates "ROX drop" and can be used when a water soluble version of ROX is required as a passive reference for normalization of fluorescent signals in real-time PCR.
