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| Calibration and Reference Dyes |
CAL Fluor® and Quasar® dye calibration standards are designed to improve the accuracy of sample signal in real-time thermal cyclers that require spectral calibration. They enable the instrument to store the fluorescent profile of each dye and control for channel cross-talk. Cross-talk is contamination of sample signal due to bleed-through from an adjacent spectral filter, an issue of particular importance in a multiplexed assay.
To achieve this, the dyes are formulated to resemble the experimental conditions. The native dye is covalently linked to the 5’end of a thymidine chain, 10 nucleotides long (T10) to better mimic the dye attached to a fluorescent probe. Using the dye calibration as a reference, the analysis software anticipates how much fluorescence to expect from each dye during amplification, and will subtract out signal from inappropriate channels. Calibration with the T10-dye standards decreases the likelihood of channel cross-talk.
CAL Fluor and Quasar dye calibration standards span a wide range of emission wavelengths to offer unsurpassed flexibility in experimental design. Because of its ubiquitous use in real-time PCR assays, we offer a FAM calibration standard. Versatile dye selection allows you to take real-time qPCR to the next level.
