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BiosearchTech: BHQ Probes used to study BRCA1 and BRCA2 mutation studies. Read the paper: http://bit.ly/cvmwRW
8/26/2010 11:06:29 AM
BiosearchTech: BHQ Probes used to study BRCA1 and BRCA2 mutation studies. Read the paper: http://bit.ly/cvmwRW
8/26/2010 11:06:29 AM
Build Your Custom Oligo
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Click on our Product Listings tab to view the synthesis scales and purification options we currently offer for custom oligonucleotide synthesis.
About the "Build Your Oligo" Quick Order
The "Build Your Oligo" Quick Order form allows you to build your custom DNA, RNA, and even DNA/RNA hybrid sequences. Since this is a purely custom order form, the price of your sequence cannot be calculated until you request a quote, or submit a shopping cart that contains your custom oligo. Our customer services department will respond shortly with pricing after having assessed your sequence.If you would like to order a dual-labeled probe, please use our Dual-Labeled Probes quick order form. We also offer other fluorogenic probe and primer formats.
A few ordering tips...
Building Oligos with RNA bases
- When entering your sequence into the Sequence Entry 5' to 3' field, indicate that your are inserting an RNA base by typing a lower case "r" in front of the base (i.e. rA, rG, rC).
Building Oligos with Internal Modification(s)
If your oligo requires an internal modification, make a selection and then click the ADD button after having typed the base positioned before the placement of your desired modification.
Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification.
Ordering Oligos Delivered in Plates
- If you would like to order plated oligos, visit our Oligos in Plates webpage where you can download our column or row format excel order forms. Prices start at $0.15 per base.
Synthesis Scale vs. Final Yield
- The synthesis scale and final yield are not the same due to the several steps involved in probe/primer synthesis. These steps include coupling of each base, cleavage of oligonucleotide from solid support and purification steps. The combination of these steps cause the final yield of the oligo to be less than the synthesis scale (starting material).
