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BiosearchTech: BHQ Probes used to study BRCA1 and BRCA2 mutation studies. Read the paper: http://bit.ly/cvmwRW
8/26/2010 11:06:29 AM
BiosearchTech: BHQ Probes used to study BRCA1 and BRCA2 mutation studies. Read the paper: http://bit.ly/cvmwRW
8/26/2010 11:06:29 AM
Scorpions® Primers
Black Hole Quencher® labeled Scorpions® primers for PCR analysis are unlike Dual-labeled probes and Molecular Beacons because they combine primer and probe in one molecule, with the primer at the 3' end and the probe contained within a hairpin-loop structure at the 5' end.
Scorpions primers utilize a uni-molecular mechanism that acts faster in solution for instantaneous fluorescence in real-time PCR. Similar to Molecular Beacons, these primers do not require enzymatic cleavage of the probe during PCR cycling. These qualities make Scorpions primers valuable tools for rapid, real-time PCR, endpoint PCR, SNP detection, gene quantification, and environmental analysis.
Scorpions primers provide a number of important advantages over other PCR probe formats:
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Scorpions Architecture
Black Hole Scorpions primers are probe/primer hybrids whose design, unlike other FRET probes, is such that they emit light only when bound to their complementary target sequence during PCR amplification. These primers incorporate two distinct structures: 1) a target-specific DNA probing sequence and 2) a target-specific PCR primer.DNA Probing Sequence
The DNA probing sequence incorporates a self-complementary stem sequence at the 3' and 5' ends that holds the amplicon specific region in a hairpin loop. A fluorophore molecule is covalently linked to the 5' end and, the dark quencher, a Black Hole Quencher dye, is linked to the 3' end of the loop structure. The fluorophore and BHQ dye are held in close physical proximity by the stem structure thereby ensuring efficient suppression of fluorescence prior to thermocycling.Target-specific Primer
The target-specific PCR primer is covalently linked to the 3' end of the DNA probing sequence through a blocking molecule. This molecule prevents polymerase read-through thereby eliminating the formation of primer dimers or mis-priming.See also:
Dual-labeled BHQ® Probes | BHQplus Probes™ | Molecular Beacons | Amplifluor® Primers | Plexor™ Primers | Dye Calibration Standards | Dyes from Biosearch Technologies
