Dual-Labeled BHQ® Probes | Biosearch Technologies

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8/26/2010 11:06:29 AM

Dual-Labeled BHQ® Probes

Dual-Labeled BHQ Probes are traditional linear, dual-labeled FRET probes incorporating a quencher and fluorophore reporter covalently linked to the 3' or 5' ends of an oligo typically 20 to 30 bases long. Fluorescence is released during the 5' exonuclease activity of Taq polymerase, which cleaves the fluorescent dye upon a probe's hybridization to its complementary sequence.

Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. Biosearch Technologies offers a wide selection of dyes for labeling Dual-Labeled BHQ Probes such as FAM, TET, HEX, JOE, TAMRA, BHQ®, and more for your real-time PCR experiment.

NOTE: Building Probes with an Internal Quencher (Currently available with FAM only)

If your probe requires an internal quencher, select your quencher, and then click the ADD button after having typed the base positioned before the placement of your desired quencher.
Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification.

Best DEAL:

$95 ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe delivered in 3-5 business days. Click on the "Order ValuProbe FAM-BHQ Probe" button on the Quick Order Form below to order online today

Instrument CALIBRATION:

FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase

Learn MORE:

Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays
The difference between FRET and static quenching

Biosearch Technologies offers RealTimeDesign™ software, a FREE qPCR assay design program hosted on the web. Start designing your Dual-Labeled BHQ Probes and primer pairs today!

Catalog #	Item Name	Price	Size/Scale	Note
DLO-RFB-5	ValuProbe™, 5' FAM/3' BHQ-1	$95.00	50 nmol	Provides 10 nmol pure.
DLO-FB1-5	Dual-labeled Probe, 5' FAM/3' BHQ-1	$150.00	100 nmol	Provides > 10 nmol pure.
DLO-FB1-2	Dual-labeled Probe, 5' FAM/3' BHQ-1	$250.00	200 nmol	Provides > 25 nmol pure.
DLO-FB1-1	Dual-labeled Probe, 5' FAM/3' BHQ-1	$365.00	1 µmol	Provides > 60 nmol pure.
DLO-F(T-B1)-2	Dual-labeled Probe, 5' FAM/Internal T-BHQ-1/3’ C3	$400.00	200 nmol	Provides > 10 nmol pure.
DLO-F(T-B1)-1	Dual-labeled Probe, 5' FAM/Internal T-BHQ-1/3’ C3	$515.00	1 µmol	Provides > 30 nmol pure.
DLO-FT-5	Dual-labeled Probe, 5' FAM/3' TAMRA	$150.00	100 nmol	Provides > 10 nmol pure.
DLO-FT-25	Dual-labeled Probe, 5' FAM/3' TAMRA	$120.00	25 nmol	Provides 5 nmol pure.
DLO-FT-2	Dual-labeled Probe, 5' FAM/3' TAMRA	$250.00	200 nmol	Provides > 25 nmol pure.
DLO-FT-1	Dual-labeled Probe, 5' FAM/3' TAMRA	$365.00	1 µmol	Provides > 60 nmol pure.
DLO-TEB1-5	Dual-labeled Probe, 5' TET/3' BHQ-1	$200.00	50 nmol	Provides > 10 nmol pure.
DLO-TEB1-25	Dual-labeled Probe, 5' TET/3' BHQ-1	$120.00	25 nmol	Provides 5 nmol pure.
DLO-TEB1-2	Dual-labeled Probe, 5' TET/3' BHQ-1	$290.00	200 nmol	Provides > 25 nmol pure.
DLO-TEB1-1	Dual-labeled Probe, 5' TET/3' BHQ-1	$420.00	1 µmol	Provides > 60 nmol pure.
DLO-TET-5	Dual-labeled Probe, 5' TET/3' TAMRA	$200.00	50 nmol	Provides > 10 nmol pure.
DLO-TET-25	Dual-labeled Probe, 5' TET/3' TAMRA	$120.00	25 nmol	Provides 5 nmol pure.
DLO-TET-2	Dual-labeled Probe, 5' TET/3' TAMRA	$295.00	200 nmol	Provides > 25 nmol pure.
DLO-TET-1	Dual-labeled Probe, 5' TET/3' TAMRA	$420.00	1 µmol	Provides > 60 nmol pure.
DLO-CGB1-5	Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1	$195.00	50 nmol	Provides > 10 nmol pure.
DLO-CGB1-25	Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1	$120.00	25 nmol	Provides 5 nmol pure.
DLO-CGB1-2	Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1	$275.00	200 nmol	Provides > 25 nmol pure.
DLO-CGB1-1	Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1	$400.00	1 µmol	Provides > 60 nmol pure.
DLO-JB1-2	Dual-labeled Probe, 5' JOE/3' BHQ-1	$450.00	200 nmol	Provides > 10 nmol pure.
DLO-JB1-1	Dual-labeled Probe, 5' JOE/3' BHQ-1	$600.00	1 µmol	Provides > 30 nmol pure.
DLO-HB1-5	Dual-labeled Probe, 5' HEX/3' BHQ-1	$205.00	50 nmol	Provides > 10 nmol pure.
DLO-HB1-25	Dual-labeled Probe, 5' HEX/3' BHQ-1	$120.00	25 nmol	Provides 5 nmol pure.
DLO-HB1-2	Dual-labeled Probe, 5' HEX/3' BHQ-1	$310.00	200 nmol	Provides > 25 nmol pure.
DLO-HB1-1	Dual-labeled Probe, 5' HEX/3' BHQ-1	$450.00	1 µmol	Provides > 60 nmol pure.
DLO-COB1-5	Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1	$195.00	50 nmol	Provides > 10 nmol pure.
DLO-COB1-25	Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1	$120.00	25 nmol	Provides 5 nmol pure.
DLO-COB1-2	Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1	$275.00	200 nmol	Provides > 25 nmol pure.
DLO-COB1-1	Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1	$400.00	1 µmol	Provides > 60 nmol pure.
DLO-Q5B2-5	Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2	$215.00	50 nmol	Provides > 10 nmol pure.
DLO-Q5B2-25	Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2	$120.00	25 nmol	Provides 5 nmol pure.
DLO-Q5B2-2	Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2	$315.00	200 nmol	Provides > 25 nmol pure.
DLO-Q5B2-1	Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2	$450.00	1 µmol	Provides > 60 nmol pure.
DLO-TB2-5	Dual-labeled Probe, 5' TAMRA/3' BHQ-2	$200.00	50 nmol	Provides > 10 nmol pure.
DLO-TB2-25	Dual-labeled Probe, 5' TAMRA/3' BHQ-2	$120.00	25 nmol	Provides 5 nmol pure
DLO-TB2-2	Dual-labeled Probe, 5' TAMRA/3' BHQ-2	$280.00	200 nmol	Provides > 25 nmol pure.
DLO-TB2-1	Dual-labeled Probe, 5' TAMRA/3' BHQ-2	$400.00	1 µmol	Provides > 60 nmol pure.
DLO-RB2-2	Dual-labeled Probe, 5' ROX/3' BHQ-2	$450.00	200 nmol	Provides > 10 nmol pure.
DLO-RB2-1	Dual-labeled Probe, 5' ROX/3' BHQ-2	$600.00	1 µmol	Provides > 30 nmol pure.
DLO-C590B2-5	Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2	$195.00	50 nmol	Provides > 10 nmol pure.
DLO-C590B2-25	Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2	$120.00	25 nmol	Provides 5 nmol pure.
DLO-C590B2-2	Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2	$270.00	200 nmol	Provides > 25 nmol pure.
DLO-C590B2-1	Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2	$390.00	1 µmol	Provides > 60 nmol pure.
DLO-CAB2-5	Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2	$215.00	50 nmol	Provides > 10 nmol pure.
DLO-CAB2-25	Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2	$120.00	25 nmol	Provides 5 nmol pure.
DLO-CAB2-2	Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2	$315.00	200 nmol	Provides > 25 nmol pure.
DLO-CAB2-1	Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2	$475.00	1 µmol	Provides > 60 nmol pure.
DLO-C635B2-5	Dual-labeled Probe, 5' CAL Fluor Red 635/3' BHQ-2	$255.00	50 nmol	Provides > 3 nmol pure.
DLO-C635B2-2	Dual-labeled Probe, 5' CAL Fluor Red 635/3' BHQ-2	$375.00	200 nmol	Provides > 10 nmol pure.
DLO-C635B2-1	Dual-labeled Probe, 5' CAL Fluor Red 635/3' BHQ-2	$550.00	1 µmol	Provides > 40 nmol pure.
DLO-B2P6-2	Dual-labeled Probe, 5’ BHQ-2/3’ Pulsar 650	$450.00	200 nmol	Provides > 10 nmol pure.
DLO-B2P6-1	Dual-labeled Probe, 5’ BHQ-2/3’ Pulsar 650	$660.00	1 µmol	Provides > 40 nmol pure.
DLO-Q6B2-5	Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2	$230.00	50 nmol	Provides 10 nmol pure.
DLO-Q6B2-25	Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2	$120.00	25 nmol	Provides 5 nmol pure.
DLO-Q6B2-2	Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2	$315.00	200 nmol	Provides > 20 nmol pure.
DLO-Q6B2-1	Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2	$475.00	1 µmol	Provides > 40 nmol pure.
DLO-Q7B2-5	Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2	$230.00	50 nmol	Provides > 3 nmol pure.
DLO-Q7B2-2	Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2	$315.00	200 nmol	Provides > 10 nmol pure.
DLO-Q7B2-1	Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2	$475.00	1 µmol	Provides > 40 nmol pure.
P2-5	Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each)	$14.00	50 nmol	Provides 20-40 nmol.
P2-2	Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each)	$25.00	200 nmol	Provides 80-160 nmol.
P2-1	Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each)	$40.00	1 µmol	Provides 400-800 nmol.
P2C-5	Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each)	$30.00	50 nmol	Provides 11.25-22.5 nmol pure.
P2C-2	Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each)	$35.00	200 nmol	Provides 45-90 nmol pure.
P2C-1	Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each)	$50.00	1 umol	Provides 225-450 nmol pure.

Dual-Labeled Probe Excel Form