Molecular Beacons | Biosearch Technologies

Forgot Log In info? Create Account.

ValuPanel™ Reagents

Dual-Labeled BHQ® Probes

Build Your Custom Oligo

Oligos for Molecular Diagnostics

RealTimeDesign™ Software

DNA/RNA Synthesis Reagents

What's new at Biosearchtech.com?

The BiosearchTech Blog

Recent Tweet...
BiosearchTech: BHQ Probes used to study BRCA1 and BRCA2 mutation studies. Read the paper: http://bit.ly/cvmwRW

8/26/2010 11:06:29 AM

Molecular Beacons

Molecular Beacons are dual-labeled FRET probes incorporating a quencher and a fluorophore reporter molecule. They differ from Dual-labeled probes, due to the incorporation of a short (~5 b.p.) complementary sequence of bases at the 3' and 5' ends. These complementary sequences hybridize to form a stem structure which holds the reporter and quencher close together in space. Also, Molecular Beacons do not rely on probe destruction from 5' exonuclease activity of Taq to generate its fluorescence, therefore, a post-PCR melt curve is possible.

Prior to amplification, the complementary bases of the stem hybridize forming the basic “stem-loop” structure of the Molecular Beacon. The stem raises the probe Tm and consequently Molecular Beacons can routinely discriminate between target sequences differing by a single nucleotide.

Multiplex assays using Molecular Beacons are now possible that can detect several targets simultaneously using multiple spectrally resolved fluorescent probes.

Molecular Beacons are sold under license from the Public Health Research Institute, Newark, NJ. For information on designing Molecular Beacons please refer to The Public Health Research Institute's Molecular Beacons website.

See also:
Dual-labeled BHQ® Probes | BHQplus Probes™ | Scorpion® Primers | Amplifluor® Primers | Plexor™ Primers | Dye Calibration Standards | Dyes from Biosearch Technologies

Catalog #	Item Name	Price	Size/Scale	Note
MBO-FB1-5	Molecular Beacon, 5' FAM/3' BHQ-1	$375.00	50 nmol	Provides > 3 nmol pure.
MBO-FB1-2	Molecular Beacon, 5' FAM/3' BHQ-1	$420.00	200 nmol	Provides > 10 nmol pure.
MBO-FB1-1	Molecular Beacon, 5' FAM/3' BHQ-1	$590.00	1 µmol	Provides > 40 nmol pure.
MBO-FD-5	Molecular Beacon, 5' FAM/3' DABCYL	$375.00	50 nmol	Provides > 3 nmol pure.
MBO-FD-2	Molecular Beacon, 5' FAM/3' DABCYL	$420.00	200 nmol	Provides > 10 nmol pure.
MBO-FD-1	Molecular Beacon, 5' FAM/3' DABCYL	$590.00	1 µmol	Provides > 40 nmol pure.
MBO-TEB1-5	Molecular Beacon, 5' TET/3' BHQ-1	$400.00	50 nmol	Provides > 3 nmol pure.
MBO-TEB1-2	Molecular Beacon, 5' TET/3' BHQ-1	$450.00	200 nmol	Provides > 10 nmol pure.
MBO-TEB1-1	Molecular Beacon, 5' TET/3' BHQ-1	$630.00	1 µmol	Provides > 40 nmol pure.
MBO-TED-5	Molecular Beacon, 5' TET/3' DABCYL	$400.00	50 nmol	Provides > 3 nmol pure.
MBO-TED-2	Molecular Beacon, 5' TET/3' DABCYL	$450.00	200 nmol	Provides > 10 nmol pure.
MBO-TED-1	Molecular Beacon, 5' TET/3' DABCYL	$630.00	1 µmol	Provides > 40 nmol pure.
MBO-CGB1-5	Molecular Beacon, 5' CAL Fluor Gold 540/3' BHQ-1	$390.00	50 nmol	Provides > 2 nmol pure.
MBO-CGB1-2	Molecular Beacon, 5' CAL Fluor Gold 540/3' BHQ-1	$435.00	200 nmol	Provides > 10 nmol pure.
MBO-CGB1-1	Molecular Beacon, 5' CAL Fluor Gold 540/3' BHQ-1	$615.00	1 µmol	Provides > 30 nmol pure.
MBO-CGD-5	Molecular Beacon, 5' CAL Fluor Gold 540/3' DABCYL	$390.00	50 nmol	Provides > 2 nmol pure
MBO-CGD-2	Molecular Beacon, 5' CAL Fluor Gold 540/3' DABCYL	$435.00	200 nmol	Provides > 10 nmol pure.
MBO-CGD-1	Molecular Beacon, 5' CAL Fluor Gold 540/3' DABCYL	$615.00	1 µmol	Provides > 30 nmol pure.
MBO-JB1-2	Molecular Beacon, 5' JOE/3' BHQ-1	$500.00	200 nmol	Provides > 10 nmol pure.
MBO-JB1-1	Molecular Beacon, 5' JOE/3' BHQ-1	$700.00	1 µmol	Provides > 30 nmol pure.
MBO-JD-2	Molecular Beacon, 5' JOE/3' DABCYL	$500.00	200 nmol	Provides > 10 nmol pure.
MBO-JD-1	Molecular Beacon, 5' JOE/3' DABCYL	$700.00	1 µmol	Provides > 30 nmol pure.
MBO-HB1-5	Molecular Beacon, 5' HEX/3' BHQ-1	$410.00	50 nmol	Provides > 2 nmol pure.
MBO-HB1-2	Molecular Beacon, 5' HEX/3' BHQ-1	$460.00	200 nmol	Provides > 10 nmol pure.
MBO-HB1-1	Molecular Beacon, 5' HEX/3' BHQ-1	$645.00	1 µmol	Provides > 30 nmol pure.
MBO-HD-5	Molecular Beacon, 5' HEX/3' DABCYL	$410.00	50 nmol	Provides > 2 nmol pure.
MBO-HD-2	Molecular Beacon, 5' HEX/3' DABCYL	$460.00	200 nmol	Provides > 10 nmol pure.
MBO-HD-1	Molecular Beacon, 5' HEX/3' DABCYL	$645.00	1 µmol	Provides > 30 nmol pure.
MBO-COB1-5	Molecular Beacon, 5' CAL Fluor Orange 560/3' BHQ-1	$390.00	50 nmol	Provides > 2 nmol pure.
MBO-COB1-2	Molecular Beacon, 5' CAL Fluor Orange 560/3' BHQ-1	$435.00	200 nmol	Provides > 10 nmol pure.
MBO-COB1-1	Molecular Beacon, 5' CAL Fluor Orange 560/3' BHQ-1	$615.00	1 µmol	Provides > 30 nmol pure.
MBO-Q5D-5	Molecular Beacon, 5' Quasar 570/3' DABCYL	$410.00	50 nmol	Provides > 3 nmol pure.
MBO-Q5D-2	Molecular Beacon, 5' Quasar 570/3' DABCYL	$460.00	200 nmol	Provides > 10 nmol pure.
MBO-Q5D-1	Molecular Beacon, 5' Quasar 570/3' DABCYL	$645.00	1 µmol	Provides > 40 nmol pure.
MBO-COD-5	Molecular Beacon, 5' CAL Fluor Orange 560/3' DABCYL	$390.00	50 nmol	Provides > 2 nmol pure.
MBO-COD-2	Molecular Beacon, 5' CAL Fluor Orange 560/3' DABCYL	$435.00	200 nmol	Provides > 10 nmol pure.
MBO-COD-1	Molecular Beacon, 5' CAL Fluor Orange 560/3' DABCYL	$615.00	1 µmol	Provides > 30 nmol pure.
MBO-Q5B2-5	Molecular Beacon, 5' Quasar 570/3' BHQ-2	$410.00	50 nmol	Provides > 3 nmol pure.
MBO-Q5B2-2	Molecular Beacon, 5' Quasar 570/3' BHQ-2	$460.00	200 nmol	Provides > 10 nmol pure.
MBO-Q5B2-1	Molecular Beacon, 5' Quasar 570/3' BHQ-2	$645.00	1 µmol	Provides > 40 nmol pure.
MBO-TB2-5	Molecular Beacon, 5' TAMRA/3' BHQ-2	$390.00	50 nmol	Provides > 2 nmol pure.
MBO-TB2-2	Molecular Beacon, 5' TAMRA/3' BHQ-2	$435.00	200 nmol	Provides > 10 nmol pure.
MBO-TB2-1	Molecular Beacon, 5' TAMRA/3' BHQ-2	$615.00	1 µmol	Provides > 30 nmol pure.
MBO-TD-5	Molecular Beacon, 5' TAMRA/3' DABCYL	$390.00	50 nmol	Provides > 2 nmol pure.
MBO-TD-2	Molecular Beacon, 5' TAMRA/3' DABCYL	$435.00	200 nmol	Provides > 10 nmol pure.
MBO-TD-1	Molecular Beacon, 5' TAMRA/3' DABCYL	$615.00	1 µmol	Provides > 30 nmol pure.
MBO-C590B2-5	Molecular Beacon, 5' CAL Fluor Red 590/3' BHQ-2	$380.00	50 nmol	Provides > 2 nmol pure.
MBO-C590B2-2	Molecular Beacon, 5' CAL Fluor Red 590/3' BHQ-2	$425.00	200 nmol	Provides > 10 nmol pure.
MBO-C590B2-1	Molecular Beacon, 5' CAL Fluor Red 590/3' BHQ-2	$605.00	1 µmol	Provides > 30 nmol pure.
MBO-RB2-2	Molecular Beacon, 5' ROX/3' BHQ-2	$500.00	200 nmol	Provides > 10 nmol pure.
MBO-RB2-1	Molecular Beacon, 5' ROX/3' BHQ-2	$700.00	1 µmol	Provides > 30 nmol pure.
MBO-RD-2	Molecular Beacon, 5' ROX/3' DABCYL	$500.00	200 nmol	Provides > 10 nmol pure.
MBO-RD-1	Molecular Beacon, 5' ROX/3' DABCYL	$700.00	1 µmol	Provides > 30 nmol pure.
MBO-CAB2-5	Molecular Beacon, 5' CAL Fluor Red 610/3' BHQ-2	$415.00	50 nmol	Provides > 2 nmol pure.
MBO-CAB2-2	Molecular Beacon, 5' CAL Fluor Red 610/3' BHQ-2	$465.00	200 nmol	Provides > 10 nmol pure.
MBO-CAB2-1	Molecular Beacon, 5' CAL Fluor Red 610/3' BHQ-2	$650.00	1 µmol	Provides > 30 nmol pure.
MBO-CAD-5	Molecular Beacon, 5' CAL Fluor Red 610/3' DABCYL	$415.00	50 nmol	Provides > 2 nmol pure.
MBO-CAD-2	Molecular Beacon, 5' CAL Fluor Red 610/3' DABCYL	$465.00	200 nmol	Provides > 10 nmol pure.
MBO-CAD-1	Molecular Beacon, 5' CAL Fluor Red 610/3' DABCYL	$650.00	1 µmol	Provides > 30 nmol pure.
MBO-C635B2-5	Molecular Beacon, 5' CAL Fluor Red 635/3' BHQ-2	$415.00	50 nmol	Provides > 2 nmol pure.
MBO-C635B2-2	Molecular Beacon, 5' CAL Fluor Red 635/3' BHQ-2	$510.00	200 nmol	Provides > 10 nmol pure.
MBO-C635B2-1	Molecular Beacon, 5' CAL Fluor Red 635/3' BHQ-2	$650.00	1 µmol	Provides > 30 nmol pure.
MBO-Q6B2-5	Molecular Beacon, 5' Quasar 670/3' BHQ-2	$415.00	50 nmol	Provides > 2 nmol pure.
MBO-Q6B2-2	Molecular Beacon, 5' Quasar 670/3' BHQ-2	$465.00	200 nmol	Provides > 10 nmol pure.
MBO-Q6B2-1	Molecular Beacon, 5' Quasar 670/3' BHQ-2	$650.00	1 µmol	Provides > 30 nmol pure.
MBO-B2P6-5	Molecular Beacon, 5' BHQ-2/3' Pulsar 650	$500.00	50 nmol	Provides > 2 nmol pure.
MBO-B2P6-2	Molecular Beacon, 5' BHQ-2/3' Pulsar 650	$550.00	200 nmol	Provides > 10 nmol pure.
MBO-B2P6-1	Molecular Beacon, 5' BHQ-2/3' Pulsar 650	$800.00	1 µmol	Provides > 30 nmol pure.
MBO-Q7B2-5	Molecular Beacon, 5' Quasar 705/3' BHQ-2	$415.00	50 nmol	Provides > 2 nmol pure.
MBO-Q7B2-2	Molecular Beacon, 5' Quasar 705/3' BHQ-2	$465.00	200 nmol	Provides > 10 nmol pure.
MBO-Q7B2-1	Molecular Beacon, 5' Quasar 705/3' BHQ-2	$650.00	1 µmol	Provides > 30 nmol pure.
P2-5	Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each)	$14.00	50 nmol	Provides 20-40 nmol.
P2-2	Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each)	$25.00	200 nmol	Provides 80-160 nmol.
P2-1	Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each)	$40.00	1 µmol	Provides 400-800 nmol.
P2C-5	Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each)	$30.00	50 nmol	Provides 11.25-22.5 nmol pure.
P2C-2	Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each)	$35.00	200 nmol	Provides 45-90 nmol pure.
P2C-1	Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each)	$50.00	1 umol	Provides 225-450 nmol pure.

Molecular Beacons Excel Form