CAL Fluor® Dyes

Historically, there was a limited selection of dyes available for multiplex PCR: TET, JOE, VIC™, HEX, Texas Red®, Cy™3, and Cy5. In recent years, the selection and quality of reporters and quenchers has dramatically improved. Biosearch Technologies developed the CAL Fluor Dyes, a set of fluorescent dyes specifically designed for qPCR instruments. These novel xanthene fluorophores not only replace previous dyes as a lower-cost alternative, they have unique patented features. These dyes can be efficiently manufactured and remain stable through oligo synthesis and work up. The attachment chemistry linking the CAL Fluor Dyes to biomolecules eliminates the problem of multiple isomers. This results in dye labels that are easier to manufacture, have a single RP-HPLC peak and have well-defined emission spectra.

CAL Fluor Dyes are available as dye-CPGs and as dye-phosphoramidites. These reactive forms allow the CAL Fluor Dyes to be incorporated during oligo synthesis, which results in more efficient label incorporation and fewer purification steps than post-synthesis labeling. The CAL Fluor Dyes have emission maxima from 520 nm to 635 nm and can be paired with BHQ-1 or BHQ-2 for efficient quenching in a variety of probe formats. Multiplex suggestions and PCR instrument compatibility of CAL Fluor Dyes can be found here.

Carboxylic acid and succinimidyl esters of the CAL Fluor Dyes are available to use for peptide labeling.

**CAL Fluor Dye Properties**
Label	Abs Max	Em Max	Dye Replaced
CAL Fluor Green 520*	500 nm	522 nm
CAL Fluor Gold 540	522 nm	544 nm	VIC, TET JOE
CAL Fluor Orange 560	538 nm	559 nm	VIC, HEX JOE
CAL Fluor Red 590	569 nm	591 nm	TAMRA
CAL Fluor Red 610	590 nm	610 nm	Texas Red®
CAL Fluor Red 615	595 nm	615 nm
CAL Fluor Red 635	618 nm	637 nm	LC RED 640®

* For peptide labeling only.

Download our Black Hole Quencher and Dye Selection Chart for more details about other offered dyes.

See Also: Black Hole Quencher® Dyes | Quasar® Dyes | Pulsar® 650 Dyes