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Support FAQs Custom Oligo Synthesis Does my oligonucleotide have a phosphate on the 5’ or 3’ end?
Support FAQs Custom Oligo Synthesis Does my oligonucleotide have a phosphate on the 5’ or 3’ end?
Does my oligonucleotide have a phosphate on the 5’ or 3’ end? |
All of our standard custom oligonucleotides are synthesized with a hydroxyl group on both the 3’ and the 5’ ends. If requested, a 5’ and/or 3’ phosphate modification can be synthesized. Dual-labeled fluorescent probes will not be extended by polymerase activity so long as there is a quencher at the 3’ end. Probes with internal quenchers require the addition of a blocker to the 3’ end. A 3’ Spacer-3 (Sp3) modification is recommended to eliminate undesired extension.
