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Mon, 15 Mar 2010 17:58:41 +0000
DNA Synthesis Reagents

Which synthesis column will work best with my machine?
Please find our column selection guide PDF:

/download/support/bti_column_selection_guide.pdf

I am getting low coupling efficiency with amidites, any suggestions?
There are two factors that can affect the coupling efficiency, amount of time and dryness of the acetonitrile. It may be helpful to increase the coupling time and if you believe the quality of the acetonitrile may have been compromised use molecular sieves to make sure it is anhydrous.