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BiosearchTech: Biosearch's Dir of GMP Mfg speaks in GEN's article: Transitioning Oligonucleotides from RUO to IVD http://t.co/A6pKrQev @GENbio
5/17/2012 4:05:05 PM
BiosearchTech: Biosearch's Dir of GMP Mfg speaks in GEN's article: Transitioning Oligonucleotides from RUO to IVD http://t.co/A6pKrQev @GENbio
5/17/2012 4:05:05 PM
How do I calibrate my instrument for the CAL Fluor Dyes? |
In order to successfully use dual-labeled probes containing CAL Fluor® and/or Quasar® dyes for real-time PCR and quantitative PCR (qPCR) multiplex assays, certain real-time PCR instruments need to be calibrated to recognize the pure dye spectra. Spectral calibration is critical for multiplexed assays to differentiate overlapping fluorescent signals from one another. The document below with give users step-by-step instructions for calibrating their instruments:
www.biosearchtech.com/assets/BTI_Spectral_Calibration_Instructions.pdf
