How do I know what fluorophore to pick for my Dual-labeled probe?

The choice of fluorescent reporter to label your dual-labeled probe(s) depends upon your instrument optics and also the degree of multiplexing you wish to achieve.

If your assays will be amplified separately and not combined together into a multiplexed arrangement, it would be recommended to label each with FAM. FAM is the most commonly used fluorophore and is nicely detected by all real-time PCR instruments.

The optic capabilities of the instrument principally determines the degree of multiplexing and which fluorophores should be used. To determine your selection, visit our Multiplexing qPCR application's webpage.

www.biosearchtech.com/assets/bti_bhq_selectionchart.pdf

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