How are fluorescent labels on dual-labeled probes accounted for when measuring the absorbance?

Biosearch calculates the extinction coefficient on labeled oligos by adding a correction factor to the extinction coefficient to account for each fluorescent label. The extinction coefficient found on the certificate of analysis accompanying any oligo purchased from Biosearch takes any dyes into consideration. Using this extinction coefficient with an OD reading at 260 should give you an accurate absorbance value; this is the way that Biosearch measures the absorbance during QC. If you require further information, we can provide you with details on what values we use for the correction factors of the dyes, but in order to simply obtain an accurate absorbance value, the extinction coefficient listed on the certificate of analysis should be sufficient.
If you need us to re-send the certificate of analysis for any of your oligos, please contact our Technical Support department at techsupport@biosearchtech.com

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