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Support FAQs qPCR Assay Design & Optimization What is the Failure Count Data in RealTimeDesign?
Support FAQs qPCR Assay Design & Optimization What is the Failure Count Data in RealTimeDesign?
What is the Failure Count Data in RealTimeDesign? |
During the primer and probe design process, the RealTimeDesign (RTD) Software uses a variety of parameter sets (e.g. Amplicon Length, Primer Tm, GC Percent) in order to design primers and probes. Each parameter set has a minimum and maximum setting. If the design falls outside of these parameters, a number will show up in the Failure Count Data indicating how many designs failed for that particular parameter. Editing the Parameters can help to alleviate the failure count.
