I am a beginner at Real Time PCR, do you have any information to help me design my Dual-labeled assay?

There are a few reference articles and resources for information on designing Dual-labeled assays. These articles have general recommendations on oligonucleotide design, cycling parameters and set-up suggestions.
http://dorakmt.tripod.com/genetics/realtime.html

Here’s the link to another helpful resource:
http://www.gene-quantification.info/ This site offers many of great links to articles on Real Time PCR.

Another great resource is the Yahoo! QPCR listserver:
http://groups.yahoo.com/group/qpcrlistserver/

Also, note that we now offer a user-friendly assay software called Real Time Design. It’s available on our website, free of charge. For information please inquiry at support@biosearchtech.com

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